Metode: Metode penelitian yang dilakukan adalah penelitian deskriptif dan penelitian eksperimental dengan rancangan post test only control group design. Tujuan penelitian ini untuk mengetahui profil kromatogram ekstrak rimpang etanol rimpang Bengle dan aktivitasnya terhadap Escherichia coli. Dalam tanaman ini diduga terdapat zat anti bakteri sehingga dimungkinkan untuk digunakan sebagai pengganti antibiotika konvensional. Latar belakang: Bengle (Zingiber cassumunar Roxb.) adalah tanaman yang sudah lama digunakan di masyarakat sebagai obat tradisional. Keywords: Ethanol extract of Bengle rhizome, Escherichia coli, anti-bacteria activity in vitro Etanol extract of Bengle rhizome had minimum consentration inhibitory 12.5% and minimum bactericide consentration 25% against Escherichia coli. No bacterial growth at etanol extract of Bengle rhizome 12.5% on Mueller Hinton Broth media, nor at consentration of 25% on Mac Conkey media.Ĭonclusions: The were 4 compounds predictly included of flavonoid, steroid, volatile oil and terpenoid group. Results: The results showed thad, thin layer chromatography shown 4 spot under UV light 254 nm wave leng with colour, blue violet, blue violet, brown yellow, brown yellow and Rf value 0.21 0.34 0.45 0.55. Chromatogram profile was using thin layer chromatography and antibacterial activity against escherichia coli was done using dilution method and than cultured in Mac Conkey agar media. Ethanol extract of Bengle rhizome was made by maceration method with ethanol as solvent. Methods: The method of these research were descriptive and experimental study using the post test only control group design. The aims of this research was to explore the chromatogram and antibacterial activity etanol extract of Bengle Rhizome against Escherichia coli. It was predicted that there were anti-bacteria substances in bengle, so it might substitutes the utilization of conventional antibiotics. Chromatogram profile and antibacterial activity of ethanol extract from bengle rhizome (Zingiber cassumunar Roxb.) to Escherichia coli in vitroīackground: Bengle (Zingiber cassumunar roxb.) was a plant which had been used by society since long time ago as a traditional medicine.
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